Research| Volume 49, ISSUE 1, P134-139, January 2023

A Single-Center Prospective Study of the Effects of Different Methods of Phlebotomy in the Emergency Department on Blood Sample Hemolysis Rates

Published:September 19, 2022DOI:



      Hemolysis is more commonly seen in the emergency department and causes delays in diagnosis, hospitalization, discharge, and treatment of patients. The aim of this study was to determine the most appropriate phlebotomy method and device to reduce blood sample hemolysis in the emergency department.


      This prospective, comparative descriptive study involved patients who presented to the emergency department with any medical condition and required blood sampling. Patients were divided into 6 groups according to the method of phlebotomy and the device used for phlebotomy. Data were analyzed with logistic regression.


      A total of 715 patients participated in the study. The blood sample hemolysis rate in the emergency department was 25.7%. When the hemolysis rates were compared with a steel straight needle or intravenous catheter, it was found that the use of steel straight needle significantly reduced hemolysis. Blood drawing through a 20 G intravenous catheter with Luer-Lock access device reduces the risk of hemolysis. Male sex and difficult blood collection also have been shown to increase the risk of hemolysis.


      Blood should be drawn with a steel straight needle instead of an intravenous catheter. However, when that is not possible, we recommend the use of a 20 G intravenous catheter with Luer-Lock access device if a blood sample is to be drawn from intravenous line.

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      Süleyman Ersoy is an Assistant Professor, Faculty of Medicine, Department of Emergency Medicine, Kırşehir Ahi Evran University, Kırşehir, Turkey. ORCID identifier:


      Bilal Ilanbey is an Assistant Professor, Faculty of Medicine, Department of Medical Biochemistry, Kırşehir Ahi Evran University, Kırşehir, Turkey. ORCID identifier: